microBrain® Assay Ready

microBrain® Assay Ready

Structure Leads to Improved Cell-Based Assay Outcomes

A Structured Environment for Cell Performance

StemoniX® microBrain® Assay Ready, pre-plated product is a human iPSC-derived, balanced culture of cortical neurons and astrocytes (nominally 50%-50%) derived from a single source that demonstrates accelerated features of cell maturity, cell functionality and cell-to-cell interaction to closely represent human brain tissue.

microBrain cortical neurons are primarily excitatory (glutamatergic) neurons balanced with inhibitory (GABAergic) neurons (approx. 80% to 20% ratio).

microBrain Assay Ready is delivered to the customer pre-plated, under ambient conditions and is an ideal model for neurotoxicity and neuro safety studies, high throughput screening and drug discovery.

Neural Network Structure & Cell Maturity

The microBrain neural network structure develops extensively and supports enhanced maturity of the neurons and astrocytes. The effectiveness of microBrain’s network structure and cell maturity level is evident by:

  • Density of Functional Synapses Resulting in Active Neuronal Circuitry
  • Expression of Key Neuronal Genes
  • Strong Response to Neurotransmitters

MAP2GFAPNuclei

Extensive Network

MAP2SynapsinNuclei

Well-Developed Synapses

Gene Expression Profile Characterized in StemoniX microBrain Neural Cultures

Glutamate Dose-Response Curve 8 weeks old

Expression of Key Neural Genes

Responsive to Neurotransmitters

Neuron & Astrocyte Advantages

The relationship between neurons and astrocytes is well documented in literature and the benefits are now available in a pre-plated format via StemoniX microBrain Assay Ready products. Astrocytes are known to support, protect and enhance the development of neurons.  microBrain’s neurons have a fully developed foundation of astrocytes that have been derived from a single source resulting in a truly physiological relevant neural model.

microBrain Demonstrates Effectiveness as a Screening Model

The relationship between neurons and astrocytes is well documented in the literature. Astrocytes are known to support, protect and enhance the development and function of neurons.  The neurons in microBrain spheroids have a fully developed foundation of astrocytes that have been derived from a single source and display key markers of cellular identity, resulting in a truly physiologically relevant neural model.

  • Known compounds demonstrated functional toxicity in human neurons in-vitro
  • Human neural progenitor cells are more sensitive to toxicity compared
    to more mature cells
  • Human iPSC-derived neural cells can be a powerful platform to
    predict toxicity of drugs to the CNS

Heat map of cell viability at different stages of cortical neuronal differentiation. PrestoBlue (Thermo) was used to assess cell viability.  Heat map units in percentage of viability.

microBrain® Assay Ready

Assay Ready microplates that are pre-plated with StemoniX cells, delivered under ambient conditions and ready for assay use after a brief preparation process.

Applications of microBrain

Safety Pharmacology & Investigative Toxicology

  • Neurotoxicity screening
  • Compatible with popular cell viability and toxicity kits
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Drug Discovery & Screening

  • Mature cortical circuitry allows for the study of normal neural electrophysiological activity
  • Phenotypic screens for key structural features (e.g. formation of synapses, dendrites)

Disease Modeling

  • Study neuroinflammation with a physiologically relevant astrocyte/neuron culture mix
  • Study the effect of hypoxia on development and function of mature cortical neurons
  • Model relevant human disease by modulation of mature cortical circuitry (e.g. seizure)

Platform Compatibility

  • High Throughput Kinetic Plate Readers (e.g. FLIPR®)
  • High Content Imaging
  • Multi-Mode Plate Readers
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Ordering Information

Product Name Catalog #
microBrain Assay Ready 96-well Plate Call for Information
microBrain Assay Ready 384-well Plate BCARX-AA-0384