Functional & Mechanistic Neurotoxic Profiling Using Human iPSC-Derived Neural Spheroid 3D Cultures
Neurological disorders affect millions of people worldwide and appear to be on the rise. While the reason for this increase remains unknown, environmental factors are a suspected contributor. Hence, there is an urgent need to develop more complex, biologically relevant and predictive in vitro assays to screen potentially neurotoxic compounds. Here, we employed a human induced pluripotent stem cell (iPSC)-based 3D neural platform composed of mature cortical neurons and astrocytes. This advanced neural platform was optimized for high throughput screening in 384-well plates and displays a highly consistent, functional performance across different wells and plates. With this model, we explored the neurotoxic profile of a library of 87 unique compounds that included pharmaceutical drugs, pesticides, flame retardants, and other chemicals.